RAM009
Effect of electroacupunture on gastric mucosal intestinal
trefoil factor gene expression of stress-induced
gastric mucosal injury in rats
World Journal of Gastroenterology
ÊÀ½ç³¦Î¸²¡Ñ§ÔÓÖ¾ 2006, 12 (12)
Xi-Ping;Li;Jie;Yan;Shou-Xiang;Yi;Xiao-Rong;Chang;Ya-Ping;Lin;Zong-Bao;Yang;Ai;Huang;Rong;Hu
Abstract:To
investigate electroacupunture£¨EA£© at the acupoints
of Stomach Meridian of Foot-Yangming£¨SMFY£©, Gallbladder
Meridian of Foot-Yangming£¨SMFY£© on gastric mucosal
intestinal trefoil factor £¨ITF£© gene expression
detection in stress-induced rats with gastric
mucosal lesion, and to explore the regulatory
mechanism and significance of EA-related gastric
mucosal protective effect. METHODS£º Forty rats
were randomly divided into 4 groups£º Blank group,
Model group, Model group£«EA at acupoints of SMFY
group£¨£¢SMFY group£¢£©, and Model group£«EA at acupoints
of GMFY group£¨GMFY group£©. All rats £¨except blank
group£© were made model by water immersion and
restraint stress £¨WRS£©. Then the gastric mucosa
tissue in each rat was taken off alter assessment
of gastric mucosal lesion index£¨GUI£©, and the
expression of ITF mRNA of the tissues was detected
by reverse transcdption-polymerase chain reaction£¨RT-PCR£©
method. RESULTS£º Compared with Model group£¨S4.3¡À
1.34£©, the GUI value in SMFY group £¨31¡À2.211 decreased
significantly£¨P¡´0.01£©, so did that in GMFY group
£¨39.8¡À 1.62, P¡´0.05£©, meanwhile GUI value in SMFY
group was significantly lower than in GMFY group£¨P¡´0.01£©.
Compared with Model group £¨0.65¡À0.01£©, EA had
a tendency to improve the expression of gastric
mucosal ITFmRNA gene£º such tendency existed in
GMFY group £¨0.66¡À0.01£© but with no signficant
difference£¨P¡µ 0.05£©, in SMFY group£¨0.76¡À0.01£©
with an extremely obvious difference £¨P¡´0.01£©,
furthermore the expression in SMFY group was significantly
higher than in GMFY group £¨P¡´ 0.01£©. CONCLUSION£º
The gastric mucosal protective effect by EA at
the acupoints of SMFY and GMFY was related to
the expression variance of ITF, indicating certain
meridian specificity exists, It could be one proof
for the TCM theory £¢Relative pardcularity between
SMFY and stomach£¢
Full text (English): USD 9.80
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